Reinhardt Laboratory

Cell-Matrix Dynamics and Pathology

 

Publications

*Wozny MR, *Nelea V, Siddiqui IFS, Wanga S, de Waard V, #Strauss M, #Reinhardt DP, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2024). Microfibril-associated glycoprotein 4 forms octamers that mediate interactions with elastogenic proteins and cells. Nat Commun 15: 4015. Abstract

Dinesh NEH, Rousseau J, Mosher DF, Strauss M, Mui J, #Campeau PM, #Reinhardt DP, #Co-corresponding authors. (2024). Mutations in fibronectin dysregulate chondrogenesis in skeletal dysplasia. Cell Mol Life Sci 81: 419. Abstract

Dinesh NEH, Baratang N, Rousseau J, Mohapatra R, Li L, Mahalingam R, Tiedemann K, #Campeau PM, #Reinhardt DP, #co-corresponding authors. (2024). Fibronectin isoforms promote postnatal skeletal development. Matrix Biol 133: 86-102. Abstract

Dinesh NEH, #Campeau PM, #Reinhardt DP, #Co-corresponding authors. (2024). The integral role of fibronectin in skeletal morphogenesis and pathogenesis. Matrix Biol 134: 23-29. Abstract

de Wagenaar NP, van den Bersselaar LM, Odijk HJHM, Stefens SJM, Reinhardt DP, Roos-Hesselink JW, Kanaar R, Verhagen JMA, Brüggenwirth HT, van de Laar IMBH, van der Pluijm I, Essers J. (2024). Functional analysis of cell lines derived from SMAD3-related Loeys-Dietz syndrome patients provides insights into genotype-phenotype relation. Hum Mol Genet 33: 1090–1104. Abstract

Brizio M, Mancini M, Lora M, Joy S, Zhu S, Brilland B, Reinhardt DP, Farge D, Langlais D, Colmegna I. (2024). Cytokine priming enhances the antifibrotic effects of human adipose derived mesenchymal stromal cells conditioned medium. Stem Cell Res Ther 15: 329. Abstract

Ellis MW, Riaz M, Huang Y, Anderson CW, Hoareau M, Li X, Luo H, Lee S, Park J, Luo J, Batty LD, Huang Q, Lopez CA, Reinhardt DP, Tellides G, Qyang Y. (2024). De novo elastin assembly alleviates development of supravalvular aortic stenosis. Arterioscler Thromb Vasc Biol 44: 1674-1682. Abstract

Siddiqui IFS, Muthu ML, Reinhardt DP. (2024). Isolation and adipogenic differentiation of murine mesenchymal stem cells harvested from macrophage-depleted bone marrow and adipose tissue. Adipocyte 13: 2350751. Abstract

Zeyer KA, Bornert O, Nelea V, Bao X, Leytens A, Sharoyan S, Sengle G, Antonyan A, Bruckner-Tuderman L, Dengjel J, Reinhardt DP, Nyström A. (2024). Dipeptidyl peptidase-4-mediated fibronectin processing evokes a pro-fibrotic extracellular matrix. J Invest Dermatol in press. Abstract

Zimmermann EA, DeVet T, Cilla M, Albiol L, Kavaseri K, Andrea C, C. J, Tiedemann K, Panahifar A, Chromik R, Komarova SV, Reinhardt DP, Zaslansky P, Willie BM. (2024). Tissue material properties, whole-bone morphology and mechanical behavior in the Fbn1 C1041G/+ mouse model of Marfan Syndrome. Matrix Biol Plus 23: 100155. Abstract

Lau C, Muthu ML, Siddiqui IF, Li L, Reinhardt DP. (2023). High-fat diet has a protective sex-dependent effect on aortic aneurysm severity in a Marfan syndrome mouse model. Can J Cardiol 39: 1553-1567. Abstract

Alonso F, Yuechao D, Li L, Jahjah T, Dupuy J-W, Fremaux I, #Reinhardt DP, #Genot EM, #Co-corresponding authors. (2023). Fibrillin-1 regulates endothelial sprouting during angiogenesis. Proc Natl Acad Sci USA 120: e2221742120. Abstract

de Souza RB, Lemes RB, Foresto-Neto O, Cassiano LL, Reinhardt DP, Meek KM, Koh IHJ, Lewis PN, Pereira LV. (2023). Extracellular matrix and vascular dynamics in the kidney of a murine model for Marfan syndrome. PLoS One 18: e0285418. Abstract

Alonso F, Li L, Frémaux I, Reinhardt DP, Génot E. (2022). Fibrillin-1 regulates arteriole integrity in the retina. Biomolecules 12: 1330. Abstract

*Muthu ML, *Tiedemann K, Fradette J, #Komarova SV, #Reinhardt DP, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2022). Fibrillin-1 regulates white adipose tissue development, homeostasis, and function. Matrix Biol 110: 106-128. Abstract

Zhang R-M, Tiedemann K, Dinesh NEH, Muthu ML, Komarova S, Ramkhelawon B, Reinhardt DP. (2022). Fibrillin-1 regulated miR-122 has a critical role in thoracic aortic aneurysm formation. Cell Mol Life Sci 79: 314. Abstract

*Tiedemann K, *Muthu ML, #Reinhardt DP, #Komarova SV, *Co-first authors contributed equally to this work, #Co-corresponding authors. (2022). Male Marfan mice are predisposed to high fat diet induced obesity, diabetes, and fatty liver. Am J Physiol Cell Physiol 323: C354-C366. Abstract

Dinesh NEH, Campeau PM, Reinhardt DP. (2022). Fibronectin isoforms in skeletal development and associated disorders. Am J Physiol Cell Physiol 323: C536-C549. Abstract

Dinesh NEH, Campeau PM, Reinhardt DP. (2022). Pathomechanisms in corner fracture type spondylometaphyseal dysplasia. MRM Insights. Abstract

Mead TJ, Martin DR, Wang LW, Cain, S. A., Gulec C, Cahill E, Mauch J, Reinhardt DP, Lo CW, Baldock C, Apte SS. (2022). Proteolysis of fibrillin-2 microfibrils is essential for normal skeletal development. eLife 11: e71142. Abstract

Boizot J, Minville-Walz M, Reinhardt DP, Bouschbacher M, Sommer P, Sigaudo-Roussel D, Debret R. (2022). FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. Int J Mol Sci 23: 1824. Abstract

Camasão D, Li L, Drouin B, Lau C, Reinhardt DP, Mantovani D. (2022). Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation. In Vitro Models: 179-195. Abstract

Balic Z, Misra S, Willard B, Reinhardt DP, Apte SS, Hubmacher D. (2021). Alternative splicing of the metalloprotease ADAMTS17 spacer regulates secretion and modulates autoproteolytic activity. FASEB J 35: e21310. Abstract

Côté E, Zhang R, Kaiser N, Reinhardt DP, Martin C. (2021). Annuloaortic ectasia in a dog: long-term follow-up and immunofluorescent study. Vet Q 41: 280-291. Abstract

de Souza RB, Gyuricza IG, Cassiano LL, Farinha-Arcieri LE, Alvim Liberatore AM, Schuindt do Carmo S, Caldeira W, Cruz MV, Ribeiro AF, Tedesco RC, Reinhardt DP, Smith R, Jun Koh IH, Pereira LV. (2021). The mgΔlpn mouse model for Marfan syndrome recapitulates the ocular phenotypes of the disease. Exp Eye Res 204: 108461. Abstract

de Souza RB, Kawahara EI, Farinha-Arcieri LE, Gerdes Gyuricza I, Neofiti-Papi B, Miranda-Rodrigues M, Cruz Grecco Teixeira M, Ribeiro Fernandes G, Barbarosa Lemes R, Reinhardt DP, Gouveia CH, Pereira LV. (2021). Hyperkyphosis is not dependent on bone mass and quality in the mouse model of Marfan syndrome. Bone 152: 116073. Abstract

Shi Y, Jones W, Beatty W, Tan Q, Mecham R, Kumra H, Reinhardt DP, Gibson MA, Reilly MA, Rodriguez J, Bassnett S. (2021). Latent-transforming growth factor beta-binding protein-2 (LTBP-2) is required for longevity but not for development of zonular fibers. Matrix Biol 95: 15-31. Abstract

Sun B, Tomita B, Salinger A, Tilvawala RR, Li L, Hakami H, Liu T, Tsoyi K, Rosas IO, Reinhardt DP, Thompson PR, Ho I-C. (2021). PAD2-mediated citrullination of fibulin-5 promotes elastogenesis. Matrix Biol 102: 70-84. Abstract

Zhang RM, Zeyer KA, Odenthal N, Zhang Y, Reinhardt DP. (2021). The fibrillin-1 RGD motif post-transcriptionally regulates ERK1/2 signaling and fibroblast proliferation via miR-1208. FASEB J 35: e21598. Abstract

Boel A, Burger J, Vanhomwegen M, Beyens A, Renard M, Barnhoorn S, Casteleyn C, Reinhardt DP, Descamps B, Vanhove C, van der Pluijm I, Coucke P, Willaert A, Essers J, Callewaert B. (2020). Slc2a10 knock-out mice deficient in ascorbic acid synthesis recapitulate aspects of arterial tortuosity syndrome and display mitochondrial respiration defects. Hum Mol Genet 29: 1476-1488. Abstract

Muthu ML, Reinhardt DP. (2020). Fibrillin-1 and fibrillin-1-derived asprosin in adipose tissue function and metabolic disorders. J Cell Commun Signal 14: 159-173. Abstract

Nelea V, Reinhardt DP. (2020). Biophysical techniques to analyze elastic tissue extracellular matrix proteins. Methods Mol Biol 2043: 213-235. Abstract

Zhang R, Kumra H, Reinhardt DP. (2020). Quantification of extracellular matrix fiber systems related to ADAMTS proteins. Methods Mol Biol 2043: 237-250. Abstract

*Kumra H, *Nelea V, Hakami H, Pagliuzza A, Djokic J, Xu J, Yanagisawa H, Reinhardt DP, *Co-first authors contributed equally to this work. (2019). Fibulin-4 exerts a dual role in LTBP-4L mediated matrix assembly and function. Proc Natl Acad Sci U S A 116: 20428-20437. Abstract

*Zeyer KA, *Zhang RM, Kumra H, Hassan A, Reinhardt DP, *Co-first authors contributed equally to this work. (2019). The fibrillin-1 RGD integrin binding site regulates gene expression and cell function through microRNAs. J Mol Biol 431: 401-421. Abstract

Burger J, van Vliet N, van Heijningen P, Kumra H, Kremers G-J, Alves M, van Cappellen G, Yanagisawa H, Reinhardt DP, Kanaar R, van der Pluijm I, Essers J. (2019). Fibulin-4 deficiency differentially affects cytoskeleton structure and dynamics as well as TGFβ signaling. Cell Signal 58: 65-78. Abstract

Costantini A, Valta H, Baratang NV, Yap P, Bertola DR, Yamamoto GL, Kim CA, Chen J, Wierenga KJ, Fanning EA, Escobar L, McWalter K, McLaughlin H, Willaert R, Begtrup A, Alm JJ, Reinhardt DP, Mäkitie O, Campeau PM. (2019). Novel fibronectin mutations expand the phenotype of the FN1-subtype of spondylometaphyseal dysplasia with "corner fractures". Bone 121: 163-171. Abstract

Dinesh NEH, Reinhardt DP. (2019). Inflammation in thoracic aortic aneurysms. Herz 44: 138-146. Abstract

Kumra H, Dinesh NEH, Reinhardt DP. (2019). Lessons from tracheal tube development for understanding congenital tracheal malformations. Eur Respir J 53: 1900127. Abstract

Wang LW, Kutz WE, Mead TJ, Beene LC, Singh S, Jenkins MW, Reinhardt DP, Apte SS. (2019). Adamts10 inactivation in mice leads to persistence of ocular microfibrils subsequent to reduced fibrillin-2 cleavage. Matrix Biol 77: 117-128. Abstract

Yin X, Wanga S, Fellows AL, Barallobre-Barreiro J, Lu R, Davaapil H, Franken R, Fava M, Baig F, Skroblin P, Xing Q, Koolbergen DR, Groenink M, Zwinderman K, Balm R, de Vries CJ, Mulder BJ, Viner R, Jahangiri M, Reinhardt DP, Sinha S, de Waard V, Mayr M. (2019). Glycoproteomic analysis of the aortic extracellular matrix in Marfan patients. Arterioscler Thromb Vasc Biol 39: 1859-1873. Abstract

Camasão D, Pezzoli D, Loy C, Kumra H, Levesque L, Reinhardt DP, Candiani G, Mantovani D. (2018). Increasing cell seeding density improves elastin expression and mechanical properties in collagen gel-based scaffolds cellularized with smooth muscle cells. Biotechnol J 1700768. Abstract

Kumra H, Reinhardt DP. (2018). Fibrillins. Methods Cell Biol 143: 223-246. Abstract

Kumra H, Sabatier L, Hassan A, Sakai T, Mosher DF, Brinckmann J, Reinhardt DP. (2018). Roles of fibronectin isoforms in neonatal vascular development and matrix integrity. PLoS Biol 16: e2004812. Abstract

Lee K-WL, Gade PS, Dong L, Zhang Z, Mubin Aral A, Jin G, Ding X, Stowell CET, Umer Nisar M, Kim K, Reinhardt DP, Solari MG, Gorantla VS, Robertson AM, Wang Y. (2018). A biodegradable synthetic graft for small arteries matches the performance of autologous vein in rat carotid arteries. Biomaterials 181: 67-80. Abstract

Pezzoli D, Di Paolo J, Kumra H, Fois G, Candiani G, **Reinhardt DP, **Mantovani D, **Co-corresponding authors contributed equally to this work. (2018). Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds. Biomaterials 180: 130-142. Abstract

Regalado ES, Mellor-Crummey L, De Backer J, Braverman AC, Ades L, Benedict S, Bradley TJ, Brickner ME, Chatfield KC, Child A, Feist C, Holmes KW, Iannucci G, Lorenz B, Mark P, Morisaki T, Morisaki H, Morris SA, Mitchell AL, Ostergaard JR, Richer J, Sallee D, Shalhub S, Tekin M, Montalcino Aortic Consortium**, Estrera A, Musolino P, Yetman A, Pyeritz R, Milewicz DM, **Reinhardt DP is member of this consortium. (2018). Clinical history and management recommendations of the smooth muscle dysfunction syndrome due to ACTA2 arginine 179 alterations. Genet Med 20: 1206-1215. Abstract

Gilpin SE, Li Q, Evangelista-Leite D, Ren X, Reinhardt DP, Frey BL, Ott HC. (2017). Fibrillin-2 and Tenascin-C bridge the age gap in lung epithelial regeneration. Biomaterials 140: 212-219. Abstract

Hubmacher D, Schneider M, Berardinelli SJ, Takeuchi H, Willard B, Reinhardt DP, Haltiwanger RS, Apte SS. (2017). Unusual life cycle and impact on microfibril assembly of ADAMTS17, a secreted metalloprotease mutated in genetic eye disease. Sci Rep 7: 41871. Abstract

Lee CS, Fu H, Baratang N, Rousseau J, Kumra H, Sutton VR, Niceta M, Ciolfi A, Yamamoto G, Bertola D, Marcelis CL, Lugtenberg D, Bartuli A, Kim C, Hoover-Fong J, Sobreira N, Pauli R, Bacino C, Krakow D, Parboosingh J, Yap P, Kariminejad A, McDonald MT, Aracena MI, Lausch E, Unger S, Superti-Furga A, Lu JT, Baylor-Hopkins Center for Mendelian Genomics, Cohn DH, Tartaglia M, Lee BH, **Reinhardt DP, **Campeau PM, **Co-corresponding authors contributed equally to this work. (2017). Mutations in fibronectin cause a subtype of spondylometaphyseal dysplasia with “corner fractures”. Am J Hum Genet 101: 815-823. Abstract

Zeyer KA, Reinhardt DP. (2017). Fibrillin-1 in connective tissues. In: Marfan Syndrome. Eds. Marfan Hilfe e. V. Springer, Berlin (Germany). Chp. 13, 64-66. Abstract

Jondeau G, Ropers J, Regalado E, Braverman A, Evangelista A, Teixedo G, De Backer J, Muino-Mosquera L, Naudion S, Zordan C, Morisaki H, Morisaki Y, von Kodolitsch Y, Dupuis-Girod S, Morris SA, Jeremy R, Odent S, Ades LC, Bakshi M, Holman K, LeMaire S, Milleron O, Langeois M, Spentchian M, Aubart M, Boileau C, Pyeritz R, Milewicz DM, Montalcino Aortic Consortium**, **Reinhardt DP is member of this consortium. (2016). International Registry of Patients Carrying TGFBR1 or TGFBR2 Mutations: Results of the Montalcino Aortic Consortium. Circ Cardiovasc Genet 9: 548-558. Abstract

Kahle B, Schmidtke C, Hunzelmann N, Bartels C, Sievers HH, Reinhardt DP, Brinckmann J. (2016). The extracellular matrix signature in vein graft disease. Can J Cardiol 32: 1008.e11-7. Abstract

Kumra H, Reinhardt DP. (2016). Fibronectin-targeted drug delivery in cancer. Adv Drug Deliv Rev 97: 101-110. Abstract

Li CD, Hancock MA, Seghal P, Zhou S, Reinhardt DP, Philip A. (2016). Soluble CD109 binds TGF-ß and antagonizes TGF-ß signaling and responses. Biochem J 473: 537-547. Abstract

Loy C, Meghezi S, Lévesque L, Pezzoli D, Kumra H, Reinhardt DP, Kizhakkedathu JN, Mantovani D. (2016). A planar model of the vessel wall from cellularized collagen scaffolds: focus on cell–matrix interactions in mono-, bi- and tri-culture models. Biomater Sci 5: 153-162. Abstract

Schaefer L, Reinhardt DP. (2016). Extracellular matrix: Therapeutic tools and targets in cancer treatment. Adv Drug Deliv Rev 97: 1-3. Abstract

Guo DC, Gong L, Regalado ES, Santos-Cortez RL, Zhao R, Cai B, Veeraraghavan S, Prakash SK, Johnson RJ, Muilenburg A, Willing M, Jondeau G, Boileau C, Pannu H, Moran R, Debacker J, GenTac Investigators National Heart Lung Blood Institute Go Exome Sequencing Project, Montalcino Aortic Consortium**, Bamshad MJ, Shendure J, Nickerson DA, Leal SM, Raman CS, Swindell EC, Milewicz DM, **Reinhardt DP is member of this consortium. (2015). MAT2A mutations predispose individuals to thoracic aortic aneurysms. Am J Hum Genet 96: 170-177. Abstract

Hinderer S, Shena N, Ringuette LJ, Hansmann J, Reinhardt DP, Brucker SY, Davis EC, Schenke-Layland K. (2015). In vitro elastogenesis - Instructing human vascular smooth muscle cells to generate an elastic fiber-containing extracellular matrix scaffold. Biomed Mater 10: 034102. Abstract

Hubmacher D, Wang LW, Mecham RP, Reinhardt DP, Apte SS. (2015). Adamtsl2 deletion results in bronchial fibrillin microfibril accumulation and bronchial epithelial dysplasia: A novel mouse model providing insights on geleophysic dysplasia. Dis Model Mech 8: 487-499. Abstract

Kaur J, Reinhardt DP. (2015). Extracellular Matrix Molecules. In: Stem Cell Biology and Tissue Engineering in Dental Science. Eds. Vishwakarma A, Sharpe PT, Shi S, Wang X, Ramalingam M. Elsevier, New York. Chp. 3, 25-43. Abstract

Regalado ES, Guo DC, Prakash S, Bensend TA, Flynn K, Estrera A, Safi H, Liang D, Hyland J, Child A, Arno G, Boileau C, Jondeau G, Braverman A, Moran R, Morisaki T, Morisaki H, Montalcino Aortic Consortium**, Pyeritz R, Coselli J, LeMaire S, Milewicz DM, **Reinhardt DP is member of this consortium. (2015). Aortic disease presentation and outcome associated with ACTA2 mutations. Circ Cardiovasc Genet 8: 457-464. Abstract

Zeyer KA, Reinhardt DP. (2015). Fibrillin-containing microfibrils are key signal relay stations for cell function. J Cell Commun Signal 9: 309-325. Abstract

Zeyer KA, Reinhardt DP. (2015). Engineered mutations in fibrillin-1 leading to Marfan syndrome act at the protein and cellular levels. Mutat Res Rev 765: 7-18. Abstract

Hubmacher D, Bergeron E, Fagotto-Kaufmann C, Sakai LY, Reinhardt DP. (2014). Early fibrillin-1 assembly monitored through a modifiable recombinant cell approach. Biomacromolecules 15: 1456-1468. Abstract

Hubmacher D, Reinhardt DP, Plesec T, Schenke-Layland K, Apte SS. (2014). Human eye development is characterized by coordinated expression of fibrillin isoforms. Invest Ophthalmol Vis Sci 55: 7934-7944. Abstract

Marek I, Volkert G, Hilgers KF, Bieritz B, Rascher W, Reinhardt DP, Hartner A. (2014). Fibrillin-1 and alpha8 integrin are co-expressed in the glomerulus and interact to convey firm adhesion of mesangial cells. Cell Adh Migr 8: 389-395. Abstract

Muiznieks LD, Cirulis JT, van der Horst A, Reinhardt DP, Wuite GJL, Pomès R, Keeley FW. (2014). Modulated growth, stability and interactions of liquid-like coacervate assemblies of elastin. Matrix Biol 36: 39-50. Abstract

Murphy EC, Mörgelin M, Reinhardt DP, Björck L, Frick IM. (2014). Finegoldia magna uses its cell surface and extracellular proteins, FAF and SufA, to colonise and pass into deeper fractions of human skin. Mol Microbiol 94: 403-417. Abstract

Reinhardt DP. (2014). Microfibril-associated disorders: fibrillinopathies. J Glaucoma 23: S34-S35. Abstract

Sabatier L, Djokic J, Hubmacher D, Dzafik D, Nelea V, Reinhardt DP. (2014). Heparin/heparan sulfate controls fibrillin-1, -2 and -3 self-interactions in microfibril assembly. FEBS Lett 588: 2890-2897. Abstract

Beene LC, Wang LW, Hubmacher D, Keene DR, Reinhardt DP, Annis DS, Mosher DF, Mecham RP, Traboulsi EI, Apte SS. (2013). Non-selective assembly of fibrillin-1 and fibrillin-2 in the rodent ocular zonule and in cultured cells: Implications for Marfan syndrome. Invest Ophthalmol Vis Sci 54: 8337-8344. Abstract

Djokic J, Fagotto-Kaufmann C, Bartels R, Nelea V, Reinhardt DP. (2013). Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers. J Biol Chem 288: 22821-22835. Abstract

Hummitzsch K, Irving-Rodgers HF, Hatzirodos N, Bonner W, Sabatier L, Reinhardt DP, Sado Y, Ninomiya Y, Wilhelm D, Rodgers RJ. (2013). A new model of development of the mammalian ovary and follicles. PLoS One 8: e55578. Abstract

Sabatier L, Djokic J, Fagotto-Kaufmann C, Chen M, Annis DS, Mosher DF, Reinhardt DP. (2013). Complex contributions of fibronectin to initiation and maturation of microfibrils. Biochem J 456: 283-295. Abstract

Tiedemann K, Boraschi-Diaz I, Rajakumar I, Kaur J, Roughley P, **Reinhardt DP, **Komarova SV, **Co-senior authors contributed equally to this work. (2013). Fibrillin-1 directly regulates osteoclast formation and function by a dual mechanism. J Cell Sci 126: 4187-4194. Abstract

Votteler M, Carvaja Berrio DA, Horke A, Sabatier L, Reinhardt DP, Nsair A, Aikawa E, Schenke-Layland K. (2013). Elastogenesis at the onset of human cardiac valve development. Development 140: 2345-2353. Abstract

Bader HL, Wang LW, Ho J, Tran T, Holden P, Fitzgerald J, Atit RP, Reinhardt DP, Apte SS. (2012). A disintegrin-like and metalloprotease domain containing thrombospondin type 1 motif-like 5 (ADAMTSL5) is a novel fibrillin-1-, fibrillin-2-, and heparin-binding member of the ADAMTS superfamily containing a netrin-like module. Matrix Biol 31: 398-411. Abstract

Kaur J, Reinhardt DP. (2012). Immobilized metal affinity chromatography co-purifies TGF-beta1 with histidine-tagged recombinant extracellular proteins. PLoS One 7: e48629. Abstract

Lammers G, Roth G, Heck M, Zengerle R, Tjabringa GS, Versteeg EM, Hafmans T, Wismans R, Reinhardt DP, Verwiel ET, Verwiel E, Zeeuwen PL, Schalkwijk J, Brock R, Daamen WF, van Kuppevelt TH. (2012). Construction of a microstructured collagen membrane mimicking the papillary dermis architecture and guiding keratinocyte morphology and gene expression. Macromol Biosci 12: 675-691. Abstract

Piha-Gossack A, Sossin W, Reinhardt DP. (2012). The evolution of extracellular fibrillins and their functional domains. PLoS One 7: e33560. Abstract

Hatzirodos N, Bayne RA, Irving-Rodgers HF, Hummitzsch K, Sabatier L, Lee S, Bonner W, Gibson MA, Rainey WE, Carr BR, Mason HD, Reinhardt DP, Anderson RA, Rodgers R. (2011). Linkage of regulators of TGF-beta activity in the fetal ovary to polycystic ovary syndrome. FASEB J 25: 2256-2265. Abstract

Hubmacher D, Reinhardt DP. (2011). Microfibrils and fibrillin. In: The Extracellular Matrix: an Overview. Eds. Mecham RP. Springer, Berlin. Chp. 7, 233-265. Abstract

Hubmacher D, Sabatier L, Annis DS, Mosher DF, Reinhardt DP. (2011). Homocysteine modifies structural and functional properties of fibronectin and interferes with the fibronectin-fibrillin-1 interaction. Biochemistry 50: 5322-5332. Abstract

Kirschner R, Hubmacher D, Iyengar G, Kaur J, Fagotto-Kaufmann C, Bromme D, Bartels R, Reinhardt DP. (2011). Classical and neonatal Marfan syndrome mutations in fibrillin-1 cause differential protease susceptibilities and protein function. J Biol Chem 286: 32810-32823. Abstract

Sabatier L, Miosge N, Hubmacher D, Lin G, Davis EC, Reinhardt DP. (2011). Fibrillin-3 expression in human development. Matrix Biol 30: 43-52. Abstract

Brinckmann J, Hunzelmann N, Kahle B, Rohwedel J, Kramer J, Hubmacher D, Reinhardt DP. (2010). Enhanced fibrillin-2 expression is a general feature of wound healing and sclerosis: Potential alteration of cell attachment and storage of TGF-beta. Lab Invest 90: 739-752. Abstract

Hubmacher D, Cirulis JT, Miao M, Keeley FW, Reinhardt DP. (2010). Functional consequences of homocysteinylation of the elastic fiber proteins fibrillin-1 and tropoelastin. J Biol Chem 285: 1188-1198. Abstract

Schenke-Layland K, Xie J, Magnusson M, Angelis E, Li X, Wu K, Reinhardt DP, MacLellan WR, Hamm-Alvarez SF. (2010). Lymphocytic infiltration leads to degradation of lacrimal gland extracellular matrix structures in NOD mice exhibiting a Sjögren's syndrome-like exocrinopathy. Exp Eye Res 90: 223-237. Abstract

Hubmacher D, Reinhardt DP. (2009). One more piece in the fibrillin puzzle. Structure 7: 635-636. Abstract

Koenders MMJF, Yang L, Wismans RG, van der Werf KO, Reinhardt DP, Daamen W, Bennink ML, Dijkstra PJ, van Kuppevelt TH, Feijen J. (2009). Microscale mechanical properties of single elastic fibers: The role of fibrillin-microfibrils. Biomaterials 30: 2425-2432. Abstract

Sabatier L, Chen D, Fagotto-Kaufmann C, Hubmacher D, McKee MD, Annis DS, Mosher DF, Reinhardt DP. (2009). Fibrillin assembly requires fibronectin. Mol Biol Cell 20: 846-858. Abstract

Sabatier L, Chen D, Fagotto-Kaufmann C, Hubmacher D, McKee MD, Annis DS, Mosher DF, Reinhardt DP. (2009). Fibrillin assembly requires fibronectin - Incytes Highlight. Mol Biol Cell 20: 1119-1119. Abstract

Cirulis JT, Bellingham CM, Davis EC, Hubmacher D, Reinhardt DP, Mecham RP, Keeley FW. (2008). Fibrillins, fibulins and MAGP modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide. Biochemistry 47: 12601-12613. Abstract

Daamen WF, Nillesen STM, Wismans R, Reinhardt DP, Hafmans T, Veerkamp JH, van Kuppevelt T. (2008). A biomaterial composed of collagen and solubilised elastin enhances angiogenesis and elastic fibre formation without calcification. Tissue Eng 14: 349-360. Abstract

Fricke M, Langer C, Brunner E, Sakai LY, Füzesi L, Reinhardt DP, Quondamatteo F. (2008). Fibrillin-1 in incisional hernias. An immunohistochemical study in scar and non-scar regions of human skin and muscle fasciae. J Anat 212: 674-685. Abstract

Hubmacher D, El-Hallous EI, Nelea V, Kaartinen MT, Lee ER, Reinhardt DP. (2008). Biogenesis of extracellular microfibrils-Multimerization of the fibrillin-1 C-terminus into bead-like structures enables self-assembly. Proc Natl Acad Sci U S A 105: 6548-6553. Abstract

Kloepper JE, Tiede S, Brinckmann J, Reinhardt DP, Fässler R, Paus R. (2008). Immunophenotyping of the human bulge region: The quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. Exp Dermatol 17: 592-609. Abstract

Wilkinson GA, Schittny JC, Reinhardt DP, Klein R. (2008). Role for ephrinB2 in postnatal lung alveolar development and elastic matrix integrity. Dev Dyn 237: 2220-2234. Abstract

El-Hallous EI, Sasaki T, Hubmacher D, Getie M, Tiedemann K, Brinckmann J, Bätge B, Davis EC, Reinhardt DP. (2007). Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adapter function to tropoelastin. J Biol Chem 282: 8935-8946. Abstract

Gnainsky Y, Kushnirsky Z, Bilu G, Hagai Y, Genina O, Volpin H, Bruck R, Spira G, Nagler A, Kawada N, Yoshizato K, Reinhardt DP, Libermann TA, Pines M. (2007). Gene expression during chemically induced liver fibrosis - effect of halofuginone on TGF-beta signaling. Cell Tissue Res 328: 153-166. Abstract

Schaefer L, Tsalastra W, Babelova A, Baliova M, Minnerup J, Sorokin L, Gröne HJ, Reinhardt DP, Pfeilschifter J, Iozzo RV, Schaefer RM. (2007). Decorin-mediated regulation of fibrillin-1 in the kidney involves the IGF-I receptor and mTOR. Am J Pathol 170: 301-315. Abstract

Drumea-Mirancea M, Wessels JT, Müller CA, Essl M, Eble JA, Tolosa M, Koch M, Reinhardt DP, Sixt M, Sorokin L, Stierhof YD, Schwarz H, Klein G. (2006). Characterization of a laminin-5 containing conduit system in the human thymus - a transport system for small molecules. J Cell Sci 119: 1396-1405. Abstract

Hartner A, Schaefer L, Porst M, Cordasic N, Gabriel A, Klanke B, Reinhardt DP, Hilgers KF. (2006). Role of fibrillin-1 in hypertensive and diabetic glomerular disease. American Journal of Physiology: Renal Physiology 290: F1329-F1336. Abstract

Hubmacher D, Tiedemann K, Reinhardt DP. (2006). Fibrillins: From biogenesis of microfibrils to signaling functions. Curr Top Dev Biol 75: 93-123. Abstract

Porst M, Daniel C, Plank C, Schocklmann HO, Reinhardt DP, Hartner A. (2006). Induction and coexpression of latent transforming growth factor beta-binding protein 1 (LTBP-1) and fibrillin-1 in experimental glomerulonephritis. Nephron Exp Nephrol 102: e99-e104. Abstract

Porst M, Plank C, Bieritz B, Konik E, Fees H, Dötsch J, Hilgers KF, Reinhardt DP, Hartner A. (2006). Fibrillin-1 regulates mesangial cell attachment, spreading, migration and proliferation. Kidney Int 69: 450-456. Abstract

Reinhardt DP. (2006). Connective Tissue. In: Marfan-Syndrom - Ein Ratgeber für Patienten, Angehörige und Betreuende. Eds. Marfan Hilfe e. V. Steinkopff Verlag, Darmstadt (Germany). Chp. 6, 26-33. Abstract

Robinson P, Arteaga-Solis E, Baldock C, Collod-Beroud G, Booms P, De Paepe A, Dietz HC, Guo G, Handford PA, Judge DP, Kielty CM, Loeys B, Milewicz DM, Ney A, Ramirez F, Reinhardt DP, Tiedemann K, Whiteman P, Godfrey M. (2006). The molecular genetics of Marfan syndrome and related disorders. J Med Genet 43: 769-787. Abstract

Robinson PN, Reinhardt DP. (2006). Research Outlook. In: Marfan-Syndrom - Ein Ratgeber für Patienten, Angehörige und Betreuende. Eds. Marfan Hilfe e. V. Steinkopff Verlag, Darmstadt (Germany). Chp. 19, 143-148. Abstract

Wu J, Reinhardt DP, Batmunkh C, Lindenmaier W, Kretschmer-Kazemi Far R, Notbohm H, Hunzelmann N, Brinckmann J. (2006). Functional diversity of lysyl hydroxylase 2 in collagen synthesis of human dermal fibroblasts. Exp Cell Res 312: 3485-3494. Abstract

Booms P, Pregla R, Ney A, Barthel F, Reinhardt DP, Pletschacher A, Mundlos S, Robinson PN. (2005). RGD-containing fibrillin-1 fragments upregulate matrix metalloproteinase expression in cell culture: A potential factor in the pathogenesis of the Marfan syndrome. Hum Genet 116: 51-61. Abstract

Bouzeghrane F, Reinhardt DP, Reudelhuber T, Thibault G. (2005). Enhanced expression of fibrillin-1, a constituent of the myocardial extracellular matrix, in fibrosis. American Journal of Physiology: Heart and Circulatory Physiology 289: H982-H991. Abstract

Brinckmann J, Hunzelmann N, El-Hallous EI, Krieg T, Sakai LY, Krengel S, Reinhardt DP. (2005). Absence of autoantibodies against correctly folded recombinant fibrillin-1 protein in systemic scleroderma patients. Arthritis Res Ther 7: R1221-R1226. Abstract

Brinckmann J, Kim S, Wu J, Reinhardt DP, Batmunkh C, Metzen E, Notbohm H, Bank RA, Krieg T, Hunzelmann N. (2005). Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: Important pathogenetic steps in early and late stage of systemic scleroderma? Matrix Biol 24: 459-468. Abstract

Hubmacher D, Tiedemann K, Bartels R, Brinckmann J, Vollbrandt T, Bätge B, Notbohm H, Reinhardt DP. (2005). Modification of the structure and function of fibrillin-1 by homocysteine suggests a potential pathogenetic mechanism in homocystinuria. J Biol Chem 280: 34946-34955. Abstract

Sixt M, Kanazawa N, Selg M, Samson T, Roos G, Reinhardt DP, Pabst R, Lutz MB, Sorokin L. (2005). The conduit system transports soluble antigens from the afferent lymph to resident dendritic cells in the T cell area of the lymph node. Immunity 22: 19-29. Abstract

Tiedemann K, Sasaki T, Gustafsson E, Göhring W, Bätge B, Notbohm H, Timpl R, Wedel T, Schlötzer-Schrehardt U, Reinhardt DP. (2005). Microfibrils at basement membrane zones interact with perlecan via fibrillin-1. J Biol Chem 280: 11404-11412. Abstract

Gaber Y, Tiedemann K, Reinhardt DP, Brinckmann J. (2004). Changes in crosslinks in the collagenous connective tissue of lipodermatosclerosis. Phlebologie 33: 8-11. Abstract

Hartner A, Eifert T, Haas CS, Tuysuz C, Hilgers KF, Reinhardt DP, Amann K. (2004). Characterization of the renal phenotype in a mouse model of Marfan Syndrome. Virchows Arch 445: 382-388. Abstract

Kassner A, Tiedemann K, Notbohm H, Ludwig T, Mörgelin M, Reinhardt DP, Chu ML, Bruckner P, Grässel S. (2004). Molecular structure and interaction of recombinant human type XVI collagen. J Mol Biol 339: 835-853. Abstract

Lorena D, Darby IA, Reinhardt DP, Sapin V, Rosenbaum J, Desmouliere A. (2004). Fibrillin-1 expression in normal and fibrotic rat liver and in cultured hepatic fibroblastic cells: Modulation by mechanical stress and role in cell adhesion. Lab Invest 84: 203-212. Abstract

Schaefer L, Mihalik D, Babelova A, Krzyzankova M, Grone HJ, Iozzo RV, Young MF, Seidler DG, Lin G, Reinhardt DP, Schaefer RM. (2004). Regulation of fibrillin-1 by biglycan and decorin is important for tissue preservation in the kidney during pressure-induced injury. Am J Pathol 165: 383-396. Abstract

Tiedemann K, Bätge B, Reinhardt DP. (2004). Assembly of microfibrils. In: Marfan Syndrome: A Primer for Clinicians and Scientists. Eds. Robinson PN, Godfrey M. Landes Bioscience, Georgetown, Texas, USA. Chp., 130-142. Abstract

Vollbrandt T, Tiedemann K, El-Hallous EI, Lin G, Brinckmann J, John H, Bätge B, Notbohm H, Reinhardt DP. (2004). Consequences of cysteine mutations in calcium-binding epidermal growth factor modules of fibrillin-1. J Biol Chem 279: 32924-32931. Abstract

Charbonneau NL, Dzamba BJ, Ono RN, Keene DR, Corson GM, Reinhardt DP, Sakai LY. (2003). Fibrillins can co-assemble in fibrils, but fibrillin fibril composition displays cell-specific differences. J Biol Chem 278: 2740-2749. Abstract

Eisenbeiss C, Martinez A, Hagedorn-Greiwe M, Reinhardt DP, Bätge B, Brinckmann J. (2003). Reduced skin thickness - a "new" minor symptom of the classical and hypermobile type of Ehlers-Danlos syndrome. Br J Dermatol 149: 850-852. Abstract

Isogai Z, Ono RN, Ushiro S, Keene DR, Chen Y, Mazzieri R, Charbonneau NL, Reinhardt DP, Rifkin DB, Sakai LY. (2003). Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated protein. J Biol Chem 278: 2750-2757. Abstract

Kassner A, Hansen U, Miosge N, Reinhardt DP, Aigner T, Bruckner-Tuderman L, Bruckner P, Grässel S. (2003). Discrete integration of collagen XVI into tissue-specific collagen fibrils or beaded microfibrils. Matrix Biol 22: 131-143. Abstract

Tiedemann K, Bätge B, Reinhardt DP. (2003). Marfan-Syndrom: Eine genetische Erkrankung des mikrofibrillären Systems. Bioforum 7-8: 456-457. Abstract

Isogai Z, Aspberg A, Keene DR, Ono RN, Reinhardt DP, Sakai LY. (2002). Versican interacts with fibrillin-1 and links extracellular microfibrils to other connective tissue networks. J Biol Chem 277: 4565-4572. Abstract

Lin G, Tiedemann K, Vollbrandt T, Peters H, Bätge B, Brinckmann J, Reinhardt DP. (2002). Homo- and heterotypic fibrillin-1 and -2 interactions constitute the basis for the assembly of microfibrils. J Biol Chem 277: 50795-50804. Abstract

Piecha D, Wiberg C, Mörgelin M, Reinhardt DP, Deak F, Maurer P, Paulsson M. (2002). Matrilin-2 interacts with itself and with other extracellular matrix proteins. Biochem J 367: 715-721. Abstract

Quondamatteo F, Reinhardt DP, Charbonneau NL, Pophal G, Sakai LY, Herken R. (2002). Fibrillin-1 and fibrillin-2 in human embryonic and early fetal development. Matrix Biol 21: 637-646. Abstract

Areida SK, Reinhardt DP, Müller PK, Fietzek PP, Köwitz J, Marinkovich MP, Notbohm H. (2001). Properties of the collagen type XVII ectodomain. Evidence for N- to C-terminal triple helix folding. J Biol Chem 276: 1594-1601. Abstract

Jensen SA, Reinhardt DP, Gibson MA, Weiss AS. (2001). MAGP-1, Protein interaction studies with tropoelastin and fibrillin-1. J Biol Chem 276: 39661-39666. Abstract

Tiedemann K, Bätge B, Müller PK, Reinhardt DP. (2001). Interactions of fibrillin-1 with heparin/heparan sulfate: Implications for microfibrillar assembly. J Biol Chem 276: 36035-36042. Abstract

Winkler J, Wirbelauer C, Lünsdorf H, Reinhardt DP, Laqua H. (2001). Immunohistochemical and charge-specific localization of anionic components of aggregates on the central anterior lens capsules from individuals with Pseudoexfoliation syndrome. Graefes Arch Clin Exp Ophthalmol 239: 952-960. Abstract

Handford PA, Downing AK, Reinhardt DP, Sakai LY. (2000). Fibrillin: from domain structure to supramolecular assembly. Matrix Biol 19: 457-470. Abstract

Reinhardt DP, Gambee JE, Ono RN, Bächinger HP, Sakai LY. (2000). Initial steps in assembly of microfibrils. Formation of disulfide-cross-linked multimers containing fibrillin-1. J Biol Chem 275: 2205-2210. Abstract

Reinhardt DP, Ono RN, Notbohm H, Müller PK, Bächinger HP, Sakai LY. (2000). Mutations in calcium-binding epidermal growth factor modules render fibrillin-1 susceptible to proteolysis: A potential disease-causing mechanism in Marfan syndrome. J Biol Chem 275: 12339-12345. Abstract

Wirbelauer C, Reinhardt DP, Lünsdorf H, Weber E, Laqua H, Winkler J. (2000). Charakterisierung der extrazellulären Matrix beim Pseudoexfoliationssyndrom. Focus MUL 17: 82-87. Abstract

Lönnqvist L, Reinhardt DP, Sakai LY, Peltonen L. (1998). Evidence for furin-type activity-mediated C-terminal processing of profibrillin-1 and interference in the processing by certain mutations. Hum Mol Genet 7: 2039-2044. Abstract

Keene DR, Jordan CD, Reinhardt DP, Ridgway CC, Ono RN, Corson GM, Fairhurst M, Sussman MD, Memoli VA, Sakai LY. (1997). Fibrillin-1 in human cartilage: developmental expression and formation of special banded fibers. J Histochem Cytochem 45: 1069-1082. Abstract

Pereira L, Andrikopoulos K, Tian J, Lee SY, Keene DR, Ono RN, Reinhardt DP, Sakai LY, Jensen-Biery N, Bunton T, Dietz HC, Ramirez F. (1997). Targeting of fibrillin-1 recapitulates the vascular phenotype of Marfan syndrome in the mouse. Nat Genet 17: 218-222. Abstract

Reinhardt DP, Mechling DE, Boswell BA, Keene DR, Sakai LY, Bächinger HP. (1997). Calcium determines the shape of fibrillin. J Biol Chem 272: 7368-7373. Abstract

Reinhardt DP, Ono RN, Sakai LY. (1997). Calcium stabilizes fibrillin-1 against proteolytic degradation. J Biol Chem 272: 1231-1236. Abstract

Pfaff M, Reinhardt DP, Sakai LY, Timpl R. (1996). Cell adhesion and integrin binding to recombinant human fibrillin-1. FEBS Lett 384: 247-250. Abstract

Reinhardt DP, Keene DR, Corson GM, Pöschl E, Bächinger HP, Gambee JE, Sakai LY. (1996). Fibrillin 1: organization in microfibrils and structural properties. J Mol Biol 258: 104-116. Abstract

Reinhardt DP, Sasaki T, Dzamba BJ, Keene DR, Chu ML, Göhring W, Timpl R, Sakai LY. (1996). Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. J Biol Chem 271: 19489-19496. Abstract

Mayer U, Zimmermann K, Mann K, Reinhardt DP, Timpl R, Nischt R. (1995). Binding properties and protease stability of recombinant human nidogen. Eur J Biochem 227: 681-686. Abstract

Reinhardt DP, Chalberg SC, Sakai LY. (1995). The structure and function of fibrillin. In: The molecular biology and pathology of elastic tissues. Eds. John Wiley and Sons, Chichester, England. Chp., 128-147. Abstract

Aumailley M, Battaglia C, Mayer U, Reinhardt DP, Nischt R, Timpl R, Fox JW. (1993). Nidogen mediates the formation of ternary complexes of basement membrane components. Kidney Int 43: 7-12. Abstract

Reinhardt DP, Mann K, Nischt R, Fox JW, Chu ML, Krieg T, Timpl R. (1993). Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. J Biol Chem 268: 10881-10887. Abstract

Kübler D, Reinhardt DP, Reed J, Pyerin W, Kinzel V. (1992). Atrial natriuretic peptide is phosphorylated by intact cells through cAMP-dependent ecto-protein kinase. Eur J Biochem 206: 179-186. Abstract

Fox JW, Mayer U, Nischt R, Aumailley M, Reinhardt DP, Wiedemann H, Mann K, Timpl R, Krieg T, Engel J, Chu ML. (1991). Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV. EMBO J 10: 3137-3146. Abstract

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Research Interests

Connective tissue disorders represent a huge economic, physically debilitating, and psychosocial burden of illness. Genetic and degenerative disorders contribute to this burden. To improve this situation, basic and applied research is required on extracellular matrices (ECM), the major component of connective tissues, and their functional relationship with cells. ECM macromolecules often specifically interact to assemble into larger functional units - building blocks for tissues and organs. Such suprastructures confer structural support and provide a plethora of instructional roles for cells.

The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular system. Fibrillins, fibulins, LTBPs, fibronectin are examples of proteins that constitute the backbone or are associated with ECM fiber systems. Mutations in these components lead to a wide spectrum of heritable connective tissue disorders, including Marfan syndrome, Beal's syndrome, stiff skin syndrome, systemic sclerosis, acromelic dysplasias, familial ectopia lentis, thoracic aortic aneurysms, familial mitral valve prolapse and myxomatous valvular disease, familial tall stature, cutis laxa, retinal dystrophy, macular degeneration, and others.

The multi-disciplinary research in the Reinhardt lab is embedded in this framework, addressing mechanisms of biogenesis and function of extracellular fiber systems in normal and pathologic situations. It is expected that the information obtained from this research program will not only benefit our basic understanding of the structure and function of extracellular fiber systems, but will also help to design new preventative, diagnostic and therapeutic strategies for the associated disorders.

Lab Members

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Contact

Mailing and Courier Address:
Reinhardt Laboratory
McGill University
Strathcona Anatomy and Dentistry Building
3640 University Street, Room 1-14
Montreal, Quebec H3A 0C7
Canada

Phone, Dieter Reinhardt: 514-398-4243
Phone, Lab: 514-398-5231

Email:
Staff: firstname.lastname@mcgill.ca
Trainees: firstname.lastname@mail.mcgill.ca